Transformation of poplar (Populus alba) plastids and expression of foreign proteins in tree chloroplasts.
Identifieur interne : 003C97 ( Main/Exploration ); précédent : 003C96; suivant : 003C98Transformation of poplar (Populus alba) plastids and expression of foreign proteins in tree chloroplasts.
Auteurs : Satoru Okumura [Japon] ; Machiko Sawada ; Yong Woo Park ; Takahisa Hayashi ; Masaki Shimamura ; Hisabumi Takase ; Ken-Ichi TomizawaSource :
- Transgenic research [ 0962-8819 ] ; 2006.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Chloroplastes, Plastes, Populus.
- Gènes rapporteurs, Transfection, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- MESH :
- genetics : Chloroplasts, Plastids, Populus.
- Genes, Reporter, Plants, Genetically Modified, Transfection.
Abstract
Plastid transformation offers several unique advantages compared with nuclear genome transformation, such as high level of transgene expression within plastids, expressing multiple transgenes as operons, lack of position effect due to site-specific transgene integration, and reducing risks of gene flow via pollen due to maternal inheritance of the plastid genome. Plastid transformation has been applied to several herbal species, but as yet there are no applications to tree species. We report here the first successful plastid transformation in a tree species, Populus alba. A vector for plastid transformation of poplar (Populus alba) was constructed, which carried the spectinomycin resistance gene and the green fluorescence protein gene as marker genes. In the regenerated shoots, the site-specific integration of foreign genes and the establishment of a high homoplastomic state were confirmed. Immunoblot analysis and histological observations corroborated the accumulation of green fluorescence protein in chloroplasts. The establishment of a plastid transformation system in poplar provides a novel tool for tree biotechnology.
DOI: 10.1007/s11248-006-9009-3
PubMed: 16952016
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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uniqKey="Park Y" first="Yong Woo" last="Park">Yong Woo Park</name></author><author><name sortKey="Hayashi, Takahisa" sort="Hayashi, Takahisa" uniqKey="Hayashi T" first="Takahisa" last="Hayashi">Takahisa Hayashi</name></author><author><name sortKey="Shimamura, Masaki" sort="Shimamura, Masaki" uniqKey="Shimamura M" first="Masaki" last="Shimamura">Masaki Shimamura</name></author><author><name sortKey="Takase, Hisabumi" sort="Takase, Hisabumi" uniqKey="Takase H" first="Hisabumi" last="Takase">Hisabumi Takase</name></author><author><name sortKey="Tomizawa, Ken Ichi" sort="Tomizawa, Ken Ichi" uniqKey="Tomizawa K" first="Ken-Ichi" last="Tomizawa">Ken-Ichi Tomizawa</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2006">2006</date><idno type="RBID">pubmed:16952016</idno><idno type="pmid">16952016</idno><idno type="doi">10.1007/s11248-006-9009-3</idno><idno type="wicri:Area/Main/Corpus">003D37</idno><idno type="wicri:explorRef" wicri:stream="Main" 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619-0292</wicri:noRegion></affiliation></author><author><name sortKey="Sawada, Machiko" sort="Sawada, Machiko" uniqKey="Sawada M" first="Machiko" last="Sawada">Machiko Sawada</name></author><author><name sortKey="Park, Yong Woo" sort="Park, Yong Woo" uniqKey="Park Y" first="Yong Woo" last="Park">Yong Woo Park</name></author><author><name sortKey="Hayashi, Takahisa" sort="Hayashi, Takahisa" uniqKey="Hayashi T" first="Takahisa" last="Hayashi">Takahisa Hayashi</name></author><author><name sortKey="Shimamura, Masaki" sort="Shimamura, Masaki" uniqKey="Shimamura M" first="Masaki" last="Shimamura">Masaki Shimamura</name></author><author><name sortKey="Takase, Hisabumi" sort="Takase, Hisabumi" uniqKey="Takase H" first="Hisabumi" last="Takase">Hisabumi Takase</name></author><author><name sortKey="Tomizawa, Ken Ichi" sort="Tomizawa, Ken Ichi" uniqKey="Tomizawa K" first="Ken-Ichi" last="Tomizawa">Ken-Ichi Tomizawa</name></author></analytic><series><title level="j">Transgenic research</title><idno 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xml:lang="en"><term>Genes, Reporter</term><term>Plants, Genetically Modified</term><term>Transfection</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Gènes rapporteurs</term><term>Transfection</term><term>Végétaux génétiquement modifiés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Plastid transformation offers several unique advantages compared with nuclear genome transformation, such as high level of transgene expression within plastids, expressing multiple transgenes as operons, lack of position effect due to site-specific transgene integration, and reducing risks of gene flow via pollen due to maternal inheritance of the plastid genome. Plastid transformation has been applied to several herbal species, but as yet there are no applications to tree species. We report here the first successful plastid transformation in a tree species, Populus alba. A vector for plastid transformation of poplar (Populus alba) was constructed, which carried the spectinomycin resistance gene and the green fluorescence protein gene as marker genes. In the regenerated shoots, the site-specific integration of foreign genes and the establishment of a high homoplastomic state were confirmed. Immunoblot analysis and histological observations corroborated the accumulation of green fluorescence protein in chloroplasts. The establishment of a plastid transformation system in poplar provides a novel tool for tree biotechnology.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16952016</PMID><DateCompleted><Year>2006</Year><Month>12</Month><Day>15</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0962-8819</ISSN><JournalIssue CitedMedium="Print"><Volume>15</Volume><Issue>5</Issue><PubDate><Year>2006</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Transgenic research</Title><ISOAbbreviation>Transgenic Res</ISOAbbreviation></Journal><ArticleTitle>Transformation of poplar (Populus alba) plastids and expression of foreign proteins in tree chloroplasts.</ArticleTitle><Pagination><MedlinePgn>637-46</MedlinePgn></Pagination><Abstract><AbstractText>Plastid transformation offers several unique advantages compared with nuclear genome transformation, such as high level of transgene expression within plastids, expressing multiple transgenes as operons, lack of position effect due to site-specific transgene integration, and reducing risks of gene flow via pollen due to maternal inheritance of the plastid genome. Plastid transformation has been applied to several herbal species, but as yet there are no applications to tree species. We report here the first successful plastid transformation in a tree species, Populus alba. A vector for plastid transformation of poplar (Populus alba) was constructed, which carried the spectinomycin resistance gene and the green fluorescence protein gene as marker genes. In the regenerated shoots, the site-specific integration of foreign genes and the establishment of a high homoplastomic state were confirmed. Immunoblot analysis and histological observations corroborated the accumulation of green fluorescence protein in chloroplasts. The establishment of a plastid transformation system in poplar provides a novel tool for tree biotechnology.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Okumura</LastName><ForeName>Satoru</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Plant Research Group, Research Institute of Innovative Technology for the Earth (RITE), 9-2 Kizugawadai, Soraku-gun, Kyoto 619-0292, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sawada</LastName><ForeName>Machiko</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Park</LastName><ForeName>Yong Woo</ForeName><Initials>YW</Initials></Author><Author ValidYN="Y"><LastName>Hayashi</LastName><ForeName>Takahisa</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Shimamura</LastName><ForeName>Masaki</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Takase</LastName><ForeName>Hisabumi</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Tomizawa</LastName><ForeName>Ken-Ichi</ForeName><Initials>K</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2006</Year><Month>09</Month><Day>02</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Transgenic Res</MedlineTA><NlmUniqueID>9209120</NlmUniqueID><ISSNLinking>0962-8819</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002736" MajorTopicYN="N">Chloroplasts</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017930" 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IdType="pubmed">15980187</ArticleId></ArticleIdList></Reference><Reference><Citation>Environ Health Perspect. 1998 Aug;106 Suppl 4:1001-4</Citation><ArticleIdList><ArticleId IdType="pubmed">9703485</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Biotechnol. 2000 Jun;18(6):257-63</Citation><ArticleIdList><ArticleId IdType="pubmed">10802561</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1990 Nov;87(21):8526-30</Citation><ArticleIdList><ArticleId IdType="pubmed">11607112</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Plant Biol. 2004;55:289-313</Citation><ArticleIdList><ArticleId IdType="pubmed">15377222</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2001 Apr;125(4):1585-90</Citation><ArticleIdList><ArticleId IdType="pubmed">11299340</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Biotechnol. 2000 Mar;18(3):333-8</Citation><ArticleIdList><ArticleId IdType="pubmed">10700152</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2004 Dec;136(4):4048-60</Citation><ArticleIdList><ArticleId IdType="pubmed">15563620</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Physiol. 2003 Sep;44(9):941-51</Citation><ArticleIdList><ArticleId IdType="pubmed">14519776</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Rep. 1991 Feb;9(10):590-4</Citation><ArticleIdList><ArticleId IdType="pubmed">24220719</ArticleId></ArticleIdList></Reference><Reference><Citation>EMBO J. 1987 Nov;6(11):3233-7</Citation><ArticleIdList><ArticleId IdType="pubmed">16453804</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Biotechnol. 2000 Jul;18(7):784-8</Citation><ArticleIdList><ArticleId IdType="pubmed">10888850</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1993 Feb 1;90(3):913-7</Citation><ArticleIdList><ArticleId IdType="pubmed">8381537</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2004 Sep;136(1):2843-54</Citation><ArticleIdList><ArticleId IdType="pubmed">15347789</ArticleId></ArticleIdList></Reference><Reference><Citation>Ann Bot. 2002 Nov;90(5):559-66</Citation><ArticleIdList><ArticleId IdType="pubmed">12466096</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 1994 Feb 11;22(3):325-31</Citation><ArticleIdList><ArticleId IdType="pubmed">8127669</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Biotechnol. 1999 Sep;17(9):910-5</Citation><ArticleIdList><ArticleId IdType="pubmed">10471936</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 1995 Feb 11;23(3):464-6</Citation><ArticleIdList><ArticleId IdType="pubmed">7885842</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2001 Nov;127(3):852-62</Citation><ArticleIdList><ArticleId IdType="pubmed">11706168</ArticleId></ArticleIdList></Reference><Reference><Citation>Exp Cell Res. 1968 Apr;50(1):151-8</Citation><ArticleIdList><ArticleId IdType="pubmed">5650857</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Mol Biol. 2004 Sep;56(2):203-16</Citation><ArticleIdList><ArticleId IdType="pubmed">15604738</ArticleId></ArticleIdList></Reference><Reference><Citation>Transgenic Res. 2006 Apr;15(2):205-17</Citation><ArticleIdList><ArticleId IdType="pubmed">16604461</ArticleId></ArticleIdList></Reference><Reference><Citation>Theor Appl Genet. 1992 Jul;84(3-4):280-5</Citation><ArticleIdList><ArticleId IdType="pubmed">24203184</ArticleId></ArticleIdList></Reference><Reference><Citation>Theor Appl Genet. 2004 Aug;109(3):451-63</Citation><ArticleIdList><ArticleId IdType="pubmed">15168022</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Biotechnol. 1998 Apr;16(4):345-8</Citation><ArticleIdList><ArticleId IdType="pubmed">9555724</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Biotechnol. 2005 May;23(5):238-45</Citation><ArticleIdList><ArticleId IdType="pubmed">15866001</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Biotechnol. 2001 Jan;19(1):71-4</Citation><ArticleIdList><ArticleId IdType="pubmed">11135556</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Biotechnol. 2001 Dec;19(12):500-6</Citation><ArticleIdList><ArticleId IdType="pubmed">11711193</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Biotechnol. 1999 Aug;17(8):808-12</Citation><ArticleIdList><ArticleId IdType="pubmed">10429249</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Physiol. 2002 Dec;43(12):1518-25</Citation><ArticleIdList><ArticleId IdType="pubmed">12514249</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Ecol. 2005 Mar;14(3):689-701</Citation><ArticleIdList><ArticleId IdType="pubmed">15723661</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Opin Biotechnol. 2005 Apr;16(2):159-66</Citation><ArticleIdList><ArticleId IdType="pubmed">15831381</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 1997 Jun 27;276(5321):2039-42</Citation><ArticleIdList><ArticleId IdType="pubmed">9197266</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Biotechnol. 2001 Sep;19(9):870-5</Citation><ArticleIdList><ArticleId IdType="pubmed">11533648</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Japon</li></country></list><tree><noCountry><name sortKey="Hayashi, Takahisa" sort="Hayashi, Takahisa" uniqKey="Hayashi T" first="Takahisa" last="Hayashi">Takahisa Hayashi</name><name sortKey="Park, Yong Woo" sort="Park, Yong Woo" uniqKey="Park Y" first="Yong Woo" last="Park">Yong Woo Park</name><name sortKey="Sawada, Machiko" sort="Sawada, Machiko" uniqKey="Sawada M" first="Machiko" last="Sawada">Machiko Sawada</name><name sortKey="Shimamura, Masaki" sort="Shimamura, Masaki" uniqKey="Shimamura M" first="Masaki" last="Shimamura">Masaki Shimamura</name><name sortKey="Takase, Hisabumi" sort="Takase, Hisabumi" uniqKey="Takase H" first="Hisabumi" last="Takase">Hisabumi Takase</name><name sortKey="Tomizawa, Ken Ichi" sort="Tomizawa, Ken Ichi" uniqKey="Tomizawa K" first="Ken-Ichi" last="Tomizawa">Ken-Ichi Tomizawa</name></noCountry><country name="Japon"><noRegion><name sortKey="Okumura, Satoru" sort="Okumura, Satoru" uniqKey="Okumura S" first="Satoru" last="Okumura">Satoru Okumura</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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